Journal article
Live-Cell Nucleocytoplasmic Protein Shuttle Assay Utilizing Laser Confocal Microscopy and FRAP
Abstract
In eukaryotes, protein trafficking to and from the nucleus, or shuttling, has been demonstrated to be an important function for proteins that have vital roles in one or both subcellular compartments. Current techniques of detecting protein nuclear shuttling are extremely labor intensive and only statically visualize evidence of shuttling. Fluorescence recovery after photobleaching (FRAP), or fluorescence microphotolysis, has proven to be an …
Authors
Howell JL; Truant R
Journal
BioTechniques, Vol. 32, No. 1, pp. 80–87
Publisher
Taylor & Francis
Publication Date
January 2002
DOI
10.2144/02321st04
ISSN
0736-6205