Journal article
Validation of a single point flow cytometric assay for determining P‐glycoprotein activity in multidrug resistant cell lines
Abstract
P-glycoprotein, a transmembrane protein which acts as an energy dependent efflux pump, has been implicated as one mechanism of multidrug resistance (MDR) in human tumours. Commonly employed assays measure P-glycoprotein immunohistochemically or mdr1 messenger RNA. In this study we compared a single point flow cytometric assay for determining activity of P-glycoprotein with cellular expression of P-glycoprotein determined by Western blot. Five …
Authors
CHIN‐YEE I; CROWTHER MA; KEENEY M; WRIGHT L; DALY S
Journal
International Journal of Laboratory Hematology, Vol. 16, No. 3, pp. 261–272
Publisher
Wiley
Publication Date
September 1994
DOI
10.1111/j.1365-2257.1994.tb00419.x
ISSN
1751-5521