Förster Resonance Energy Transfer (FRET) materials play an important role for quantifying concentrations and distances in biosensing and bioimaging. 1,2 Nanoparticles, such as quantum dots (QDs), 3 upconversion nanoparticles (UCNPs), 4 or gold nanoparticles (AuNPs) 5 can significantly enhance FRET and provide unique spectral (color) and temporal (lifetime) photoluminescence properties to the FRET system. Adding the broad choice of biological recognition molecules, including antibodies, artificial protein binders, aptamers, nucleic acids, or peptides to the FRETomics toolbox provides a boundless source for designing fluorescent probes for biosensing, bioimaging, and theranostics. 6 The presentation will explain time-resolved and time-gated FRET and the specific benefits for spectral and temporal luminescence multiplexing with different luminescent and plasmonic nanomaterials. Recent applications of multiplexed FRET hybrid nanomaterials will be discussed. References (1) R. Su et al. Chem. Rev. 125 , 9429–9551 (2025). (2) R. Algar et al. Nat. Methods 16 , 815-829 (2019). (3) N. Hildebrandt et al. Chem. Rev. 117 , 536-711 (2017). (4) E. Madirov et al. Acc. Chem. Res., accepted (2025). (5) N. Hildebrandt et al. Chem. Commun. 59 , 2352-2380 (2023). (6) Check out https://nanofret.com/ for publications. Figure 1
