Journal article
PiggyBac Transposon Mutagenesis: A Tool for Cancer Gene Discovery in Mice
Abstract
Transposons are mobile DNA segments that can disrupt gene function by inserting in or near genes. Here, we show that insertional mutagenesis by the PiggyBac transposon can be used for cancer gene discovery in mice. PiggyBac transposition in genetically engineered transposon-transposase mice induced cancers whose type (hematopoietic versus solid) and latency were dependent on the regulatory elements introduced into transposons. Analysis of 63 …
Authors
Rad R; Rad L; Wang W; Cadinanos J; Vassiliou G; Rice S; Campos LS; Yusa K; Banerjee R; Li MA
Journal
Science, Vol. 330, No. 6007, pp. 1104–1107
Publisher
American Association for the Advancement of Science (AAAS)
Publication Date
November 19, 2010
DOI
10.1126/science.1193004
ISSN
0036-8075