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Characterization of anti-glycophorin monoclonal...
Journal article

Characterization of anti-glycophorin monoclonal antibodies

Abstract

This group of mouse monoclonal reagents comprised 31 different antibodies, 19 in the form of culture supernatants and 12 as ascitic fluids. The isotypes of antibodies in supernatant form were determined using a sandwich ELISA immunodot assay, before performing initial serological and physicochemical characterisation by microplate haemagglutination. Culture supernatants were titred without prior dilution, whereas ascitic fluids and purified …

Authors

Fraser RH; Murphy MT; Inglis G; Mitchell R

Journal

Transfusion Clinique et Biologique, Vol. 31, No. 2, pp. 289–296

Publisher

Elsevier

Publication Date

April 1988

DOI

10.1016/s0338-4535(88)80115-6

ISSN

1246-7820