Chapter 4 Adenoviral Vector Construction I Mammalian Systems

This chapter describes the construction of adenoviral vectors for mammalian systems. Ads are converted into mammalian gene transfer vectors by replacing the El region with the foreign DNA of interest. All methods for manipulating Ad genomes for construction of vectors rely on the observation that purified viral DNA is infectious. Early methods for generating recombinant Ad involved direct manipulation of viral DNA extracted from virions. …