Fast and High-Resolution Fractionation of Positional Isomers of a PEGylated Protein Using Membrane Chromatography

The fractionation of positional isomers of a PEGylated protein is quite challenging as these have similar molecular weight, and only very slightly different surface charge. In this study, cation exchange z2 laterally-fed membrane chromatography (z2LFMC), which has been shown to be suitable for high-speed, high-resolution protein purification, was used to fractionate positional isomers of mono-PEGylated lysozyme. The performance of the z2LFMC …