Journal article
The characterization of microsatellite loci in the communally breeding smooth‐billed ani (Crotophaga ani)
Abstract
Abstract Microsatellite DNA sequences were isolated and characterized from a Puerto Rican population of communally nesting smooth‐billed anis for use in a parentage/kinship study. Lambda Zap Express (Stratagene) was used to construct a library of 350 000 independent recombinant phage and screened with TG and AAT probes. In total, 85 positive clones (73 TG and 12 AAT) were isolated. Primer pairs were designed for 17 sequences and five …
Authors
Blanchard L; Quinn JS
Journal
Molecular Ecology Resources, Vol. 1, No. 3, pp. 152–154
Publisher
Wiley
Publication Date
September 2001
DOI
10.1046/j.1471-8278.2001.00056.x
ISSN
1755-098X