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Calculation of Cellular Oxygen Concentration for...
Journal article

Calculation of Cellular Oxygen Concentration for Photodynamic Therapy In Vitro

Abstract

In vitro photodynamic therapy experiments are usually performed by irradiating cells in confluent or nearly confluent monolayer cultures. Oxygen is consumed in the monolayer by photodynamic reactions and cellular respiration and is supplied by diffusion from the overlying medium. Calculations of oxygen concentration by numerical solution of the time-dependent diffusion equation show that hypoxia can be induced in the monolayer under typical PDT …

Authors

Patterson MS; Mazurek E

Journal

Methods in Molecular Biology, Vol. 635, , pp. 195–205

Publisher

Springer Nature

Publication Date

2010

DOI

10.1007/978-1-60761-697-9_14

ISSN

1064-3745