The use of DNPH‐derivatized protein carbonyls as a marker of oxidative stress in mouse heart and liver

Reactive oxygen species (ROS) have been shown to modify proteins, lipids and DNA. These modified biomolecules serve as markers for the quantification of oxidative stress in various physiological and diseased conditions. Identification and quantification of a marker of protein oxidation (i.e., protein carbonyls) in tissues continue to be time consuming and/or costly. To identify and quantify this biomarker, we utilized a mouse model of ALS …