Sulfation and uptake of the maturation-inducing steroid, 17α,20β-dihydroxy-4-pregnen-3-one by rainbow trout ovarian follicles

Rainbow trout ovarian follicles were incubated in vitro with tritiated 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P; maturation-inducing steroid). Within 18–24 h, 56–66% had been converted to tritiated 17α,20β-dihydroxy-4-pregnen-3-one 20-sulfate (identification confirmed by HPLC) and 27% had been taken up (absorbed) by the follicles. Addition of 125 ng of cold (non-tritiated) 17,20β-P to the incubations caused a decrease in the percentage of …