Journal article
Individually Resolved DNA Molecules Stretched and Embedded in Electrospun Polymer Nanofibers
Abstract
We have used the flow characteristics of an electrospinning jet to elongate and fix DNA molecules. We embedded and observed fluorescently labeled lambda bacteriophage DNA molecules in polyethylene oxide nanofibers. The embedded DNA molecules were imaged using fluorescence microscopy and found to be stretched to lengths approaching the full dyed contour length.
Authors
Bellan LM; Cross JD; Strychalski EA; Moran-Mirabal J; Craighead HG
Journal
Nano Letters, Vol. 6, No. 11, pp. 2526–2530
Publisher
American Chemical Society (ACS)
Publication Date
November 1, 2006
DOI
10.1021/nl061894+
ISSN
1530-6984