Journal article
The Solution Structure and Dynamics of an Arc Repressor Mutant Reveal Premelting Conformational Changes Related to DNA Binding †
Abstract
The solution structure of the hyperstable MYL mutant (R31M/E36Y/R40L) of the Arc repressor of bacteriophage P22 was determined by NMR spectroscopy and compared to that of the wild-type Arc repressor. A backbone rmsd versus the average of 0.37 A was obtained for the well-defined core region. For both Arc-MYL and the wild-type Arc repressor, evidence for a fast equilibrium between a packed ("in") conformation and an extended ("out") conformation …
Authors
Nooren IMA; Rietveld AWM; Melacini G; Sauer RT; Kaptein R; Boelens R
Journal
Biochemistry, Vol. 38, No. 19, pp. 6035–6042
Publisher
American Chemical Society (ACS)
Publication Date
May 1, 1999
DOI
10.1021/bi982677t
ISSN
0006-2960