Journal article
The Projection Analysis of NMR Chemical Shifts Reveals Extended EPAC Autoinhibition Determinants
Abstract
EPAC is a cAMP-dependent guanine nucleotide exchange factor that serves as a prototypical molecular switch for the regulation of essential cellular processes. Although EPAC activation by cAMP has been extensively investigated, the mechanism of EPAC autoinhibition is still not fully understood. The steric clash between the side chains of two conserved residues, L273 and F300 in EPAC1, has been previously shown to oppose the inactive-to-active …
Authors
Selvaratnam R; VanSchouwen B; Fogolari F; Mazhab-Jafari MT; Das R; Melacini G
Journal
Biophysical Journal, Vol. 102, No. 3, pp. 630–639
Publisher
Elsevier
Publication Date
February 2012
DOI
10.1016/j.bpj.2011.12.030
ISSN
0006-3495