Journal article
Inhibitor screening of pharmacological chaperones for lysosomal β-glucocerebrosidase by capillary electrophoresis
Abstract
Pharmacological chaperones (PCs) represent a promising therapeutic strategy for treatment of lysosomal storage disorders based on enhanced stabilization and trafficking of mutant protein upon orthosteric and/or allosteric binding. Herein, we introduce a simple yet reliable enzyme assay using capillary electrophoresis (CE) for inhibitor screening of PCs that target the lysosomal enzyme, β-glucocerebrosidase (GCase). The rate of GCase-catalyzed …
Authors
Shanmuganathan M; Britz-McKibbin P
Journal
Analytical and Bioanalytical Chemistry, Vol. 399, No. 8, pp. 2843–2853
Publisher
Springer Nature
Publication Date
March 2011
DOI
10.1007/s00216-011-4671-6
ISSN
1618-2642