Journal article
Physiological and molecular characterization of methotrexate transport by Malpighian tubules of adult Drosophila melanogaster
Abstract
A radioisotope tracer technique and quantitative PCR were used to study the mechanisms and regulation of transepithelial transport of the type II organic anion methotrexate (MTX) by the Malpighian tubules of Drosophila melanogaster. Transport of MTX was saturable and Na(+)-independent; the kinetic parameters J(max) and K(t) were 437fmolmin(-1) and 23.5microM, respectively. The transport of MTX was competitively inhibited by phenol red and …
Authors
Chahine S; O’Donnell MJ
Journal
Journal of Insect Physiology, Vol. 55, No. 10, pp. 927–935
Publisher
Elsevier
Publication Date
October 2009
DOI
10.1016/j.jinsphys.2009.06.005
ISSN
0022-1910