Journal article
General transduction in Rhizobium meliloti
Abstract
General transduction by phage phi M12 in Rhizobium meliloti SU47 and its derivatives is described. Cotransduction and selection for Tn5 insertions which are closely linked to specific loci were demonstrated. A derivative of SU47 carrying the recA::Tn5 allele of R. meliloti 102F34 could be transduced for plasmid R68.45 but not for chromosomally located alleles. Phage phi M12 is morphologically similar to Escherichia coli phage T4, and …
Authors
Finan TM; Hartweig E; LeMieux K; Bergman K; Walker GC; Signer ER
Journal
Journal of Bacteriology, Vol. 159, No. 1, pp. 120–124
Publisher
American Society for Microbiology
Publication Date
July 1984
DOI
10.1128/jb.159.1.120-124.1984
ISSN
0021-9193