Journal article
A shotgun lipidomics study of a putative lysophosphatidic acid acyl transferase (PlsC) in Sinorhizobium meliloti
Abstract
A shotgun lipidomics approach was used to study the knockout mutant of a putative lysophosphatidic acyl acid transferase (PlsC) in order to delineate the function of this enzyme in Sinorhizobium meliloti. In plsC knockout mutant lipids that contained 16:0 and 16:1 fatty acids and their biosynthetically related cyclopropane fatty acid (cis-9,10-methylene hexadecanoic acid) decreased up to 93%. Tandem mass spectrometry experiments in the presence …
Authors
Basconcillo LS; Zaheer R; Finan TM; McCarry BE
Journal
Journal of Chromatography B, Vol. 877, No. 26, pp. 2873–2882
Publisher
Elsevier
Publication Date
September 2009
DOI
10.1016/j.jchromb.2009.05.014
ISSN
1570-0232