Journal article
Determination of 4‐hydroxyproline‐2‐epimerase activity by capillary electrophoresis: A stereoselective platform for inhibitor screening of amino acid isomerases
Abstract
Isomerases involved in the metabolism of D/L-amino acids represent promising therapeutic targets for treatment of disease. Herein, we report a tunable platform for the assessment of enzymatic kinetics involving amino acid isomerization by CE that offers improved selectivity and sensitivity over traditional methods. Enzyme activity and competition assays were evaluated for various hydroxyproline diastereoisomers, proline enantiomers and their …
Authors
Gavina JMA; White CE; Finan TM; Britz‐McKibbin P
Journal
Electrophoresis, Vol. 31, No. 16, pp. 2831–2837
Publisher
Wiley
Publication Date
August 2010
DOI
10.1002/elps.201000187
ISSN
0173-0835