Journal article
Protocol to desensitize human and murine mast cells after polyclonal IgE sensitization
Abstract
In this protocol, we provide detailed instructions to desensitize human and murine mast cells (MCs) after polyclonal IgE sensitization. Moreover, we specify the steps for MC degranulation assessment after desensitization, measuring CD63 and CD107a expression by flow cytometry and β-hexosaminidase activity. Desensitized MCs can be used directly for co-culture with other cell types, immunofluorescence, live imaging, and omics approaches. For …
Authors
López-Sanz C; Sánchez-Martínez E; Jiménez-Saiz R
Journal
STAR Protocols, Vol. 3, No. 4,
Publisher
Elsevier
Publication Date
12 2022
DOI
10.1016/j.xpro.2022.101755
ISSN
2666-1667