Journal article
Testing NF-kappa B1-p50 antibody specificity using knockout mice.
Abstract
Cell extracts from knockout mice can provide definitive proof of antibody specificity. Two NF-kappa B1-p50 antibodies, sc-114 (a commercial antibody) and NR1157, were observed to recognize proteins having distinct electrophoretic mobilities of 52-55 kD and 50 kD, respectively, by Western blot analysis. In order to discriminate the specificity of these antibodies for NF-kappa B1-p50, whole cell extracts derived from NF-kappa B1-p105 knockout …
Authors
Pereira DS; Kushner DB; Ricciardi RP; Graham FL
Journal
Oncogene, Vol. 13, No. 2, pp. 445–446
Publication Date
July 18, 1996
ISSN
0950-9232