Journal article
An Enhanced System for Construction of Adenoviral Vectors by the Two-Plasmid Rescue Method
Abstract
The two-plasmid rescue method of constructing Ad vectors, which relies on either homologous or Cre-mediated recombination between two plasmids cotransfected into 293 or 293Cre4 cells, respectively, offers advantages over other approaches because of its simplicity. We have improved the efficiency of vector construction by both homologous and Cre-mediated recombination by replacing the single ITR in the shuttle plasmid with a head-to-head ITR …
Authors
Ng P; Parks RJ; Cummings DT; Evelegh CM; Graham FL
Journal
Human Gene Therapy, Vol. 11, No. 5, pp. 693–699
Publisher
Mary Ann Liebert
Publication Date
March 20, 2000
DOI
10.1089/10430340050015590
ISSN
1043-0342