Unequal α and β Globin mRNA in Reticulocytes of Normal and Mutant f/f Fetal Mice

Messenger RNA (mRNA) was isolated from adult as well as normal and mutant f/f fetal mouse reticulocytes by poly-U affinity chromatography. mRNA from normal adult reticulocytes directed equal synthesis of alpha and beta globin chains in wheat germ cell free translational system. mRNA from either normal or mutant f/f fetal reticulocytes directed 40% more alpha globin synthesis than beta globin synthesis. These observations are suggestive that there is significantly more alpha mRNA than beta mRNA in normal and mutant f/f fetal mouse reticulocytes.