Calcium influx and spermatogenesis in the testis and liver enzyme activities in the zebrafish are rapidly modulated by the calcium content of the water

This study investigated the effects of varying environmental Ca²⁺ concentrations on the influx of Ca²⁺ to the testis, testicular morphology, and liver enzymes in the zebrafish. Adult zebrafish (Danio rerio) were held in water containing low (0.02 mM), control (0. 7 mM) or high (2 mM) Ca²⁺ concentrations for 12 h. Testes were then incubated in vitro with 0.1 μCi/mL ⁴⁵Ca²⁺ to measure Ca²⁺ influx at 30 and 60 min and qualitative and quantitative …