An Improved Solid Phase Extraction Procedure for the Determination of Antidepressants in Serum by Column Liquid Chromatography

A convenient and economical procedure for the isolation of antidepressant drugs from serum is described. A 0.7 mL aliquot of the sample after the addition of trimipramine or protriptyline as the internal standard is vortex mixed with 0.7 mL of acetonitrile and centrifuged. The supernatant is applied to 1-mL BondElut C-18 silica extraction column which has been conditioned by subsequent washing with 1 M HCI, methanol, water, and 1% potassium …