Journal article
An optimized protocol for phenotyping human granulocytes by mass cytometry
Abstract
Granulocytes encompass diverse roles, from fighting off pathogens to regulating inflammatory processes in allergies. These roles are represented by distinct cellular phenotypes that we captured with mass cytometry (CyTOF). Our protocol enables simultaneous evaluation of human basophils, eosinophils, and neutrophils under homeostasis and upon immune activation by anti-Immunoglobulin E (anti-IgE) or interleukin-3 (IL-3). Granulocyte integrity and …
Authors
Gonzalez NV; Oliveria J-P; Tebaykin D; Ivison GT; Mukai K; Tsai MM; Borges L; Nadeau KC; Galli SJ; Tsai AG
Journal
STAR Protocols, Vol. 3, No. 2,
Publisher
Elsevier
Publication Date
6 2022
DOI
10.1016/j.xpro.2022.101280
ISSN
2666-1667