Journal article
Highly Purified versus Filtered Crude Collagenase: Comparable Human Islet Isolation Outcomes
Abstract
This study was designed to retrospectively compare the impact of crude Sigma V collagenase (Sigma V, n = 52) with high-purified Serva NB1 collagenase (Serva NB1, n = 42) on human islet isolation outcomes. A three-step filtration was applied to the crude Sigma V to remove endotoxin contamination and impurities; in addition, this process was used as a lot prescreening tool. Isolation outcomes were determined by digestion efficacy, islet yields, …
Authors
Wang Y; Paushter D; Wang S; Barbaro B; Harvat T; Danielson KK; Kinzer K; Zhang L; Qi M; Oberholzer J
Journal
Cell Transplantation, Vol. 20, No. 11-12, pp. 1817–1825
Publisher
SAGE Publications
Publication Date
December 2011
DOI
10.3727/096368911x564994
ISSN
0963-6897