Journal article
Optimisation of media for the culture of normal human epithelial cells from oesophageal mucosa
Abstract
A technique has been developed which allows growth and histology/cytochemistry of primary oesophageal mucosal explant cultures to be monitored over four weeks. The paper describes experiments designed to optimise media and culture conditions. The results suggest that optimal growth can be obtained in media containing RPMI 1640, and 10% horse or newborn calf serum. McCoy's 5A medium or Dulbecco's modified MEM could be substituted for RPMI but …
Authors
Mothersill C; Cusack A; Seymour CB; Hennessy TP
Journal
Cell Biology International, Vol. 13, No. 7, pp. 625–633
Publisher
Wiley
Publication Date
July 1989
DOI
10.1016/0309-1651(89)90113-6
ISSN
1065-6995