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Nontargeted Serum Lipid Profiling of Nonalcoholic...
Journal article

Nontargeted Serum Lipid Profiling of Nonalcoholic Steatohepatitis by Multisegment Injection–Nonaqueous Capillary Electrophoresis–Mass Spectrometry: A Multiplexed Separation Platform for Resolving Ionic Lipids

Abstract

New methods are needed for global lipid profiling due to the complex chemical structures and diverse physicochemical properties of lipids. Herein we introduce a robust data workflow to unambiguously select lipid features from serum ether extracts by multisegment injection-nonaqueous capillary electrophoresis-mass spectrometry (MSI-NACE-MS). An iterative three-stage screening strategy is developed for nontargeted lipid analyses when using …

Authors

Ly R; Ly N; Sasaki K; Suzuki M; Kami K; Ohashi Y; Britz-McKibbin P

Journal

Journal of Proteome Research, Vol. 21, No. 3, pp. 768–777

Publisher

American Chemical Society (ACS)

Publication Date

March 4, 2022

DOI

10.1021/acs.jproteome.1c00682

ISSN

1535-3893