Journal article
High‐Affinity Dimeric Aptamers Enable the Rapid Electrochemical Detection of Wild‐Type and B.1.1.7 SARS‐CoV‐2 in Unprocessed Saliva
Abstract
We report a simple and rapid saliva-based SARS-CoV-2 antigen test that utilizes a newly developed dimeric DNA aptamer, denoted as DSA1N5, that specifically recognizes the spike proteins of the wildtype virus and its Alpha and Delta variants with dissociation constants of 120, 290 and 480 pM, respectively, and binds pseudotyped lentiviruses expressing the wildtype and alpha trimeric spike proteins with affinity constants of 2.1 pM and 2.3 pM, …
Authors
Zhang Z; Pandey R; Li J; Gu J; White D; Stacey HD; Ang JC; Steinberg C; Capretta A; Filipe CDM
Journal
Angewandte Chemie International Edition, Vol. 60, No. 45, pp. 24266–24274
Publisher
Wiley
Publication Date
November 2, 2021
DOI
10.1002/anie.202110819
ISSN
1433-7851