Journal article
The importance of selecting the right internal control gene to study the effects of antenatal glucocorticoid administration in human placenta
Abstract
RT-qPCR requires a suitable set of internal control genes (ICGs) for an accurate normalization. The usefulness of 7 previously published ICGs in the human placenta was analyzed according to the effects of betamethasone treatment, sex and fetal age. Raw RT-qPCR data of the ICGs were evaluated using published algorithms. The algorithms revealed that a reliable normalization was achieved using the geometrical mean of PPIA, RPL19, HMBS and SDHA. …
Authors
Gütling H; Bionaz M; Sloboda DM; Ehrlich L; Braun F; Gramzow AK; Henrich W; Plagemann A; Braun T
Journal
Placenta, Vol. 44, , pp. 19–22
Publisher
Elsevier
Publication Date
August 2016
DOI
10.1016/j.placenta.2016.05.011
ISSN
0143-4004