Journal article
An efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species
Abstract
We describe an improved allelic-exchange method for generating unmarked mutations and chromosomal DNA alterations in enterobacterial species. Initially developed for use in Salmonella enterica, we have refined the method in terms of time, simplicity, and efficiency. We have extended its use into related bacterial species that are more recalcitrant to genetic manipulations, including enterohemorrhagic and enteropathogenic Escherichia coli and …
Authors
White AP; Allen-Vercoe E; Jones BW; DeVinney R; Kay WW; Surette MG
Journal
Canadian Journal of Microbiology, Vol. 53, No. 1, pp. 56–62
Publisher
Canadian Science Publishing
Publication Date
January 1, 2007
DOI
10.1139/w06-102
ISSN
0008-4166