Clonal tracking of hESCs reveals differential...

Clonal tracking of hESCs reveals differential contribution to functional assays

Abstract

Retroviral integration is used to mark clones in human embryonic stem cell cultures and clonal distribution is assessed after functionally testing the cells with different methods. Distinct subsets of clones are detected after in vitro differentiation versus teratoma formation in vivo.

Authors

Stewart MH; Bendall SC; Levadoux-Martin M; Bhatia M

Journal

Nature Methods, Vol. 7, No. 11, pp. 917–922

Publisher

Springer Nature

Publication Date

January 1, 2010

DOI

10.1038/nmeth.1519

ISSN

1548-7091

Associated Experts

Mick Bhatia

Professor, Faculty of Health Sciences

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