Chapter 53 Construction and Propagation of Human Adenovirus Vectors

This chapter describes methods for inserting foreign genes into the adenoviruses (Ad) genome and for purifying, growing, and titrating the recombinant viruses. Adenoviruses (Ads), which have been used extensively as a model system for molecular studies of mammalian cell DNA replication, transcription, and RNA processing, are now being increasingly investigated as potential mammalian expression vectors for gene therapy and for recombinant …