Voltage-gated calcium channels regulate K+ transport in the Malpighian tubules of the larval cabbage looper, Trichoplusia ni

Transporting epithelia are tissues that specialize in the directional movements of ions and water and are typically either secretory or reabsorptive. Recent work on the Malpighian tubule of larval lepidopterans (caterpillars) demonstrated that the distal ileac plexus segment of this epithelium is capable of rapidly switching between ion secretion and reabsorption. Subsequent transcriptomic studies suggested expression of voltage-gated ion channels in the lepidopteran MTs (which are not contractile and not innervated). The present study shows that isolated MTs of larval Trichoplusia ni express α1, β2, and α2δ4 subunits of voltage-gated Ca²⁺ channel Ca_V1 and that pan-Ca_Vα immunoreactivity is present in the apical and basolateral membranes of the principal cells. Basolateral membrane potential (V_bl) in isolated MTs of larval Trichoplusia ni was influenced by Ca_V1 functioning; pharmacological inhibition of Ca_V1 reversed V_bl from inside-negative to inside-positive, and also reduced transepithelial potential (V_te), lowered [Ca²⁺]_i and reversed the direction of K⁺ transport from secretion to reabsorption. Thus, our findings indicate that a functional Ca_V1 channel is necessary for constitutive K⁺ secretion observed in isolated preparations of lepidopteran MTs. Lastly, V_te and V_bl of isolated MTs were influenced by changes in bathing saline [K⁺]. Our findings suggest that epithelia may rely on Ca_V channels to enable robust ion secretion and downregulation of Ca_V channels, together with other transcriptional changes, enables ion reabsorption.