Journal article
A Highly Specific DNA Aptamer for RNase H2 from Clostridium difficile
Abstract
Molecular recognition elements with high specificity are of great importance for the study of molecular interactions, accurate diagnostics, drug design, and personalized medicine. Herein, a highly specific DNA aptamer for RNase H2 from Clostridium difficile (C. difficile) was generated by SELEX and minimized to 40 nucleotides. The aptamer exhibits a dissociation constant (Kd) of 1.8 ± 0.5 nM and an inhibition constant (IC50) of 7.1 ± 0.6 nM for …
Authors
Li J; Gu J; Zhang H; Liu R; Zhang W; Mohammed-Elsabagh M; Xia J; Morrison D; Zakaria S; Chang D
Journal
ACS Applied Materials & Interfaces, Vol. 13, No. 8, pp. 9464–9471
Publisher
American Chemical Society (ACS)
Publication Date
March 3, 2021
DOI
10.1021/acsami.0c20277
ISSN
1944-8244