Journal article
Accurate flow cytometric membrane potential measurement in bacteria using diethyloxacarbocyanine and a ratiometric technique
Abstract
BACKGROUND: Membrane potential (MP) plays a critical role in bacterial physiology. Existing methods for MP estimation by flow cytometry are neither accurate nor precise, due in part to the heterogeneity of size of the particles analyzed. The ratio of a size- and MP-sensitive measurement, and an MP-independent, size-sensitive measurement, should provide a better estimate of MP.
METHODS: Flow cytometry and spectrofluorometry were used to detect …
Authors
Novo D; Perlmutter NG; Hunt RH; Shapiro HM
Journal
Cytometry, Vol. 35, No. 1, pp. 55–63
Publisher
Wiley
Publication Date
January 1, 1999
DOI
10.1002/(sici)1097-0320(19990101)35:1<55::aid-cyto8>3.0.co;2-2
ISSN
0196-4763