Journal article
Estrogen Receptor-&agr; and Endothelial Nitric Oxide Synthase Nuclear Complex Regulates Transcription of Human Telomerase
Abstract
We report that in endothelial cells, the angiogenic effect of 17beta-estradiol (E2) is inhibited by the estrogen receptor (ER) antagonist ICI or the NO synthase (NOS) inhibitor 7-nitroindazole via downregulation of hTERT, the telomerase catalytic subunit, suggesting that E2 and NO are involved in controlling hTERT transcription. Quantitative Real-Time PCR and chromatin immunoprecipitations in E2-treated human umbilical vein endothelial cells, …
Authors
Grasselli A; Nanni S; Colussi C; Aiello A; Benvenuti V; Ragone G; Moretti F; Sacchi A; Bacchetti S; Gaetano C
Journal
Circulation Research, Vol. 103, No. 1, pp. 34–42
Publisher
Wolters Kluwer
Publication Date
July 3, 2008
DOI
10.1161/circresaha.107.169037
ISSN
0009-7330
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Active Transport, Cell NucleusAmino Acid SubstitutionAnimalsCattleCell NucleusCells, CulturedDNA MethylationEndothelial CellsEnzyme InhibitorsEstradiolEstrogen Receptor alphaEstrogensGene Expression Regulation, EnzymologicHumansIndazolesMiceMice, KnockoutMutation, MissenseNitric Oxide Synthase Type IINitric Oxide Synthase Type IIIResponse ElementsTelomeraseTranscription, GeneticTransfectionUmbilical Veins