Journal article
Nonisotopic Detection of Human Papillomavirus DNA in Clinical Specimens Using a Consensus PCR and a Generic Probe Mix in an Enzyme-Linked Immunosorbent Assay Format
Abstract
We assessed the value of a new digoxigenin (DIG)-labeled generic probe mix in a PCR-enzyme-linked immunosorbent assay format to screen for the presence of human papillomavirus (HPV) DNA amplified from clinical specimens. After screening with this new generic assay is performed, HPV DNA-positive samples can be directly genotyped using a reverse blotting method with product from the same PCR amplification. DNA from 287 genital specimens was …
Authors
Kornegay JR; Shepard AP; Hankins C; Franco E; Lapointe N; Richardson H; Coutleé F
Journal
Journal of Clinical Microbiology, Vol. 39, No. 10, pp. 3530–3536
Publisher
American Society for Microbiology
Publication Date
10 2001
DOI
10.1128/jcm.39.10.3530-3536.2001
ISSN
0095-1137