Nuclear localizing sequences promote nuclear translocation and enhance the radiotoxicity of the anti-CD33 monoclonal antibody HuM195 labeled with 111In in human myeloid leukemia cells.

Abstract

Our objective was to evaluate the toxicity of the anti-CD33 monoclonal antibody HuM195 modified with peptides (CGYGPKKKRKVGG) harboring the nuclear localizing sequence (NLS; underlined) of simian virus 40 large T antigen and labeled with (111)In against acute myeloid leukemia (AML) cells. METHODS: HuM195 was derivatized with sulfosuccinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate (sulfo-SMCC) to introduce maleimide groups for reaction …

Authors

Chen P; Wang J; Hope K; Jin L; Dick J; Cameron R; Brandwein J; Minden M; Reilly RM

Journal

Journal of Nuclear Medicine, Vol. 47, No. 5, pp. 827–836

Publication Date

May 2006

ISSN

0161-5505

Associated Experts

Kristin Hope

Associate Professor (Part-Time), Faculty of Health Sciences

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Fields of Research (FoR)

32 Biomedical and Clinical Sciences 3202 Clinical sciences

Medical Subject Headings (MeSH)

Active Transport, Cell Nucleus Antibodies, Monoclonal Antigens, CD Antigens, Differentiation, Myelomonocytic Cell Proliferation Cell Survival HL-60 Cells Humans Indium Radioisotopes K562 Cells Leukemia, Myeloid Nuclear Localization Signals Peptides Radionuclide Imaging Sialic Acid Binding Ig-like Lectin 3 U937 Cells