Journal article
Metagenomics to Paleogenomics: Large-Scale Sequencing of Mammoth DNA
Abstract
We sequenced 28 million base pairs of DNA in a metagenomics approach, using a woolly mammoth (Mammuthus primigenius) sample from Siberia. As a result of exceptional sample preservation and the use of a recently developed emulsion polymerase chain reaction and pyrosequencing technique, 13 million base pairs (45.4%) of the sequencing reads were identified as mammoth DNA. Sequence identity between our data and African elephant (Loxodonta africana) …
Authors
Poinar HN; Schwarz C; Qi J; Shapiro B; MacPhee RDE; Buigues B; Tikhonov A; Huson DH; Tomsho LP; Auch A
Journal
Science, Vol. 311, No. 5759, pp. 392–394
Publisher
American Association for the Advancement of Science (AAAS)
Publication Date
January 20, 2006
DOI
10.1126/science.1123360
ISSN
0036-8075