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A multiplexed confocal FLIM microscope with 4-taps...
Conference

A multiplexed confocal FLIM microscope with 4-taps time-gated imager

Abstract

Combined with confocal imaging, Fluorescence lifetime imaging microscopy (FLIM) can achieve 3-dimensional optical sectional capability with sub-nanosecond lifetime information. As confocal FLIM acquires multi-dimensional data 4D (3D space + time), it is inherently slow. Recent developments in lock-in pixel imagers with time gated pixels show such detectors are capable of collecting as many as 8-time gates in a single pixel cycle. We present a …

Authors

Richards M; Shirakawa Y; Badr F; Kagawa K; Kawahito S; Fang Q

Volume

11245

Publisher

SPIE, the international society for optics and photonics

Publication Date

February 17, 2020

DOI

10.1117/12.2550208

Name of conference

Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVII

Conference proceedings

Progress in biomedical optics and imaging

ISSN

1605-7422