Biopolymers and synthetic polymers for precise quantification

Precise quantification of biopolymer and synthetic polymers is very important for biomaterials research. Radio-labeling and gamma counter readings provide 50-100 times greater sensitivity than that of UV and florescence determination methods. The radioiodine labeling method has been well established for protein quantification through iodination of phenol groups of tyrosine within the protein structure[l]. The aim of this work is to modify and label biopolymers such as hyaluronic acid (HA) for studying its interaction with phenolboronic acid(PBA) and synthetic polymers such as polyvinyl amine (PVAm) electrolyte for biosensor paper preparation and PLGA-PNIPAAm block copolymer for ophthalmic drug delivery by incorporation of a phenol group into these polymer chains. Thus, these polymers can be labeled using ICL method to achieve precise quantification. The method allows not only accurate quantification, but also ease of imaging to investigate the distribution of the polymer.