Protein bioseparation by membrane chromatography using polyelectrolyte gelcoated adsorptive membranes

The use of polyelectrolyte gel coated adsorptive membranes for protein bioseparation by membrane chromatography was discussed. The protein binding capacity of the polyelectrolyte gel-coated, ion-exchange membrane was significantly higher than that reported in literature. The results show that in the pH range, 5.5-7.5, dynamic adsorption of human serum albumin (HSA) increased with increase in pH. It was also found that the amount of HSA bound was protein concentration independent in the range studied.