Journal article
Unraveling Determinants of Affinity Enhancement in Dimeric Aptamers for a Dimeric Protein
Abstract
High-affinity aptamers can be derived de novo by using stringent conditions in SELEX (Systematic Evolution of Ligands by EXponential enrichment) experiments or can be engineered post SELEX via dimerization of selected aptamers. Using electrophoretic mobility shift assays, we studied a series of heterodimeric and homodimeric aptamers, constructed from two DNA aptamers with distinct primary sequences and secondary structures, previously isolated …
Authors
Manochehry S; McConnell EM; Li Y
Journal
Scientific Reports, Vol. 9, No. 1,
Publisher
Springer Nature
DOI
10.1038/s41598-019-54005-4
ISSN
2045-2322