Journal article
Purification of silica‐free DNA and characterization of its role in coagulation
Abstract
BACKGROUND: Although extracellular DNA has been reported to activate coagulation, its direct effects and consequent interpretations have recently been questioned because of silica and polyphosphate (polyP) contaminations when DNA is isolated using common silica-based kits.
OBJECTIVES: To identify and characterize alternative methods of isolating DNA that is free of silica with functionally undetectable levels of polyP.
Authors
Medeiros SK; Zafar N; Liaw PC; Kim PY
Journal
Journal of Thrombosis and Haemostasis, Vol. 17, No. 11, pp. 1860–1865
Publisher
Elsevier
Publication Date
11 2019
DOI
10.1111/jth.14565
ISSN
1538-7933