Nondestructive cryomicro-CT imaging enables structural and molecular analysis of human lung tissue

Micro-computed tomography (CT) enables three-dimensional (3D) imaging of complex soft tissue structures, but current protocols used to achieve this goal preclude cellular and molecular phenotyping of the tissue. Here we describe a radiolucent cryostage that permits micro-CT imaging of unfixed frozen human lung samples at an isotropic voxel size of (11 µm)³ under conditions where the sample is maintained frozen at -30°C during imaging. The …