Journal article
A simplified method for preparing IVF granulosa cells for culture
Abstract
PURPOSE: To validate an expedited method for the removal of erythrocytes when preparing IVF granulosa-luteal cells for culture. METHODS: Granulosa cells were enriched for culturing from follicular aspirates by density gradient centrifugation and by hypo-osmotic lysis treatments. RESULTS: Cells prepared by either method showed the same cell viability and produced progesterone in similar quantities. CONCLUSIONS: Using hypo-osmotic lysis to remove …
Authors
Lobb DK; Younglai EV
Journal
Journal of Assisted Reproduction and Genetics, Vol. 23, , pp. 93–95
Publication Date
February 2006
DOI
10.1007/s10815-006-9025-5
ISSN
1058-0468