Development of a rainbow trout intestinal epithelial cell line and its response to lipopolysaccharide

A cell line, RTgutGC, was developed from the intestine of Oncorhynchus mykiss. RTgutGC has an epithelial‐like shape, been passaged over 100 times, and cryopreserved successfully. A rainbow trout origin was confirmed by sequencing a 652 bp region of the mitochondrial cytochrome c oxidase I gene. RTgutGC is grown routinely in Leibovitz’s L15 without glutamine supplemented with 10% fetal bovine serum (FBS). Cell viability was evaluated with Alamar …