Localization of constitutive and hyperthermia‐inducible heat shock mRNAS (hsc70 and hsp70) in the rabbit cerebellum and brainstem by non‐radioactive in situ hybridization

Neural expression of constitutive hsc70 mRNA and hyperthermia-inducible hsp70 mRNA is examined using radioactive and non-radioactive in situ hybridization procedures. A strong induction of hsp70 mRNA was noted in cell populations in cerebellar layers and in the brainstem which demonstrated expression of mRNA encoding proteolipid protein, an oligodendrocyte marker. The non-radioactive in situ hybridization procedure using digoxigenin …